Cholangiocarcinoma (CCA) comprises a heterogeneous group of biliary cancers with poor prognosis. These tumors are usually asymptomatic and are consequently often diagnosed at advanced stages, when the disease is already widespread, severely limiting the assess to the available potential curative options. The KLF transcription factor family regulates important physiological and pathological processes, by modulating the expression of genes involved in distinct signaling pathways. Numerous studies have demonstrated the critical role of some members of this family in the pathogenesis of various cancers, with some being considered as potential diagnostic/prognostic tools and therapeutic targets. Yet, the role of KLFs in CCA remains an unexplored subject. Particularly, KLF15, is tightly connected with liver metabolic function and differentiation and its dysregulation was previously reported in distinct types of cancer, yet the role of KLF15 in CCA remains an unexplored subject. For this reason, this dissertation aims to explore the role of KLF15 in cholangiocarcinogenesis and evaluate its potential as a diagnostic, prognostic and therapeutic tool.