08/05/17

03/05/17

Fecha / Data: Lunes, 8 de mayo de 2017 / 2017ko maiatzak 8, Astelehena

Hora / Ordua: 13:30

Lugar / Lekua: IIS Biodonostia, Sala de Formación, Planta 3ª / Biodonostia OIIko Formakuntza Aretoa, 3.Solairua

Organizador/ Antolatzailea: Cell Signaling Technology (Werfem)

Para una mejor organización del evento, se ruega confirmación de asistencia antes del 3 de Mayo.

Para aquellos que deseen plantear alguna duda previa al curso, tendrán a su disposición al responsables de Werfen en la zona adjunta a la Sala de Formación desde las 11:00h.

 

Sriram Aravamudhan, PhD  – Field Technology and Product Manager  – Cell Signaling Technology

Immunofluorescence (IF) combines the use of antibodies with fluorescence imaging techniques to visualizetarget proteins and other biomolecules within fixed cell or tissue samples. This process can reveal thelocalization, relative expression, and even activation states of target proteins. When performing IFexperiments, proteins of interest can be detected using either primary antibodies covalently conjugated tofluorophores (direct detection) or a two-step approach with unlabeled primary antibody followed by fluorophoreconjugatedsecondary antibody (indirect detection). At Cell Signaling Technology (CST), our goal is to provide highly specific antibodies that yield strong, specific signal with minimal background. Our scientists screen a large number of antibodies and recommend only those best suited for the application. In this seminar, we will discuss the critical steps for a successful immunofluorescence experiment, and our validation efforts on extensive protocol optimization and antibody titration to determine the best working conditions for each antibody, providing supporting data to explain our recommendations. We will also introduce tyramide signal amplification, a system that allows fluorescent detection of multiple protein markers in FFPE tissues (referred to as multiplex immunohistochemistry).

 

About The speaker: Sriram Aravamudhan

Sriram Aravamudhan received his Master of Science in Drug Discovery and Translational Biology from the University of Edinburgh, UK and then moved to the Max Planck Institute for Heart and Lung Research in Germany where he pursued his doctoral research in the department of cardiac development and remodeling and worked on a novel component of the mitochondrial energetics complex in heart. He gained extensive experience in mass-spectrometry based proteomics as well as diverse biochemical applications during his PhD. In September 2015, Sriram started working at Cell Signaling Technology as a Field Application Specialist to support scientists with appropriate research tools and continues to do so in his transformed role as a Field Technology and Product Manager since the beginning of 2017.